fhuC [2017-11-16 15:26:57]

hydroxamate siderophore ABC transporter (ATP-binding protein) (ferrichrome und ferrioxamine)

Locus: BSU33290

Isoelectric point: 6.50

Molecular weight: 29.80 kDa

Protein length: 269 aa

Gene length: 807 bp

Function: siderophore uptake

Product: hydroxamate siderophore ABC transporter (ATP-binding protein) (ferrichrome und ferrioxamine)

Essential: no

E.C.: 3.6.3.34

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of iron/ siderophores
• Cellular processes → Homeostasis → Acquisition of iron → ABC transporters for the uptake of iron/ siderophores
• Metabolism → Additional metabolic pathways → Iron metabolism → ABC transporters for the uptake of iron/ siderophores
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,415,387 → 3,416,196

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + iron chelate(Out) = ADP + phosphate + iron chelate(In) (according to Swiss-Prot)

Structure

• 4R9U, the E. coli BtuC-BtuD complex, BtuD shares 34% identity, 55% similarity with FhuC, PubMed

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: fhuB-fhuG-fhuC

  Description

  • PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed

Biological materials

Mutant

• BKE33290 (ΔfhuC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCGGCCCCCTCCTCT, downstream forward: _UP4_TGAAAAAATCCCGGTTTGAA
• BKK33290 (ΔfhuC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCGGCCCCCTCCTCT, downstream forward: _UP4_TGAAAAAATCCCGGTTTGAA

References

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